{"id":19680,"date":"2022-03-22T16:11:44","date_gmt":"2022-03-22T15:11:44","guid":{"rendered":"https:\/\/www.idisantiago.es\/plataformas-de-apoyo-comun\/citometria-de-flujo\/"},"modified":"2022-06-06T09:40:01","modified_gmt":"2022-06-06T07:40:01","slug":"citometria-de-flujo","status":"publish","type":"page","link":"https:\/\/www.idisantiago.es\/en\/plataformas-de-apoyo-comun\/citometria-de-flujo\/","title":{"rendered":"Flow cytometry"},"content":{"rendered":"<p>[et_pb_section fb_built=&#8221;1&#8243; admin_label=&#8221;T\u00edtulo&#8221; _builder_version=&#8221;4.14.1&#8243; _module_preset=&#8221;default&#8221; custom_margin=&#8221;64px||0px||false|false&#8221; custom_padding=&#8221;0px||||false|false&#8221; da_disable_devices=&#8221;off|off|off&#8221; locked=&#8221;off&#8221; collapsed=&#8221;on&#8221; global_colors_info=&#8221;{}&#8221; da_is_popup=&#8221;off&#8221; da_exit_intent=&#8221;off&#8221; da_has_close=&#8221;on&#8221; da_alt_close=&#8221;off&#8221; da_dark_close=&#8221;off&#8221; da_not_modal=&#8221;on&#8221; da_is_singular=&#8221;off&#8221; da_with_loader=&#8221;off&#8221; da_has_shadow=&#8221;on&#8221;][et_pb_row _builder_version=&#8221;4.10.7&#8243; _module_preset=&#8221;default&#8221; width=&#8221;100%&#8221; custom_padding=&#8221;0px||||false|false&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column type=&#8221;4_4&#8243; _builder_version=&#8221;4.10.7&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_post_title meta=&#8221;off&#8221; featured_image=&#8221;off&#8221; _builder_version=&#8221;4.14.1&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][\/et_pb_post_title][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=&#8221;1&#8243; _builder_version=&#8221;4.10.7&#8243; _module_preset=&#8221;default&#8221; custom_padding=&#8221;0px||||false|false&#8221; da_disable_devices=&#8221;off|off|off&#8221; global_colors_info=&#8221;{}&#8221; da_is_popup=&#8221;off&#8221; da_exit_intent=&#8221;off&#8221; da_has_close=&#8221;on&#8221; da_alt_close=&#8221;off&#8221; da_dark_close=&#8221;off&#8221; da_not_modal=&#8221;on&#8221; da_is_singular=&#8221;off&#8221; da_with_loader=&#8221;off&#8221; da_has_shadow=&#8221;on&#8221;][et_pb_row _builder_version=&#8221;4.10.7&#8243; _module_preset=&#8221;default&#8221; width=&#8221;100%&#8221; custom_padding=&#8221;0px||||false|false&#8221; locked=&#8221;on&#8221; collapsed=&#8221;on&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column type=&#8221;4_4&#8243; _builder_version=&#8221;4.10.7&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_text _builder_version=&#8221;4.14.7&#8243; _dynamic_attributes=&#8221;link_option_url&#8221; _module_preset=&#8221;default&#8221; link_option_url=&#8221;@ET-DC@eyJkeW5hbWljIjp0cnVlLCJjb250ZW50IjoicG9zdF9saW5rX3VybF9wYWdlIiwic2V0dGluZ3MiOnsicG9zdF9pZCI6Ijk1MDAifX0=@&#8221; global_colors_info=&#8221;{}&#8221;]COMMON SUPPORT PLATFORMS[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=&#8221;2_3,1_3&#8243; _builder_version=&#8221;4.10.7&#8243; _module_preset=&#8221;default&#8221; width=&#8221;100%&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column type=&#8221;2_3&#8243; _builder_version=&#8221;4.10.7&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_toggle title=&#8221;Equipment&#8221; admin_label=&#8221;Equipamiento&#8221; _builder_version=&#8221;4.14.8&#8243; _module_preset=&#8221;default&#8221; body_font=&#8221;|300|||||||&#8221; hover_enabled=&#8221;0&#8243; global_colors_info=&#8221;{}&#8221; sticky_enabled=&#8221;0&#8243;]<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-15067 size-medium alignleft\" src=\"https:\/\/www.idisantiago.es\/wp-content\/uploads\/2022\/03\/IMG_20220301_160251-300x138.jpg\" alt=\"Citometro-cellsorter\" width=\"300\" height=\"138\" \/><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-15064 size-medium alignnone\" src=\"https:\/\/www.idisantiago.es\/wp-content\/uploads\/2022\/03\/IMG_20220301_160235_2-300x121.jpg\" alt=\"citometro-cytek\" width=\"300\" height=\"121\" \/>\u200b<\/p>\n<p><strong>FACSAria IIu sorter<\/strong>\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0<strong>Cytek Northern Lights<\/strong><\/p>\n<p>La Unidad se complementa con cabinas de flujo laminar, incubadores, centr\u00edfugas, microscop\u00eda de fluorescencia y confocal, lo que permite a los usuarios realizar dentro de la Unidad la mayor\u00eda de sus experimentos, evitando problem\u00e1ticas de viabilidad celular en el caso de sorting.<\/p>\n<p>[\/et_pb_toggle][et_pb_toggle title=&#8221;Description&#8221; admin_label=&#8221;Descripci\u00f3n&#8221; _builder_version=&#8221;4.14.8&#8243; _module_preset=&#8221;default&#8221; body_font=&#8221;|300|||||||&#8221; global_colors_info=&#8221;{}&#8221;]The cytometry-sorter platform of the Santiago de Compostela Health Research Institute (IDIS) is a unique scientific-technological infrastructure that provides service to all IDIS research groups, as well as Galician universities and the regional biotechnology sector.<\/p>\n<p>Flow cytometry is a cell analysis technique that allows the light scattering and fluorescence characteristics of cells to be measured when they pass through a beam of light. The main objectives of this platform are:<\/p>\n<ul>\n<li>To advise IDIS users on the principles and applications of flow cytometry analysis and cell sorting.<\/li>\n<li>To develop, optimize and carry out new analytical applications demanded by researchers and users.<\/li>\n<li>To do cell isolation through cell sorting.<br \/>\nQuantify different soluble cytokines using multiplex tests.<\/li>\n<\/ul>\n<p>[\/et_pb_toggle][et_pb_toggle title=&#8221;Services&#8221; admin_label=&#8221;Servicios&#8221; _builder_version=&#8221;4.14.7&#8243; _module_preset=&#8221;default&#8221; body_font=&#8221;|300|||||||&#8221; global_colors_info=&#8221;{}&#8221;]The FACSAria IIu cytometry-sorting platform allows the analysis of up to 9 parameters of particles between 0.5 and 100 \u00b5m, with a maximum speed of 70,000 events per second. This makes it possible to identify different cell populations within the same sample, determine the presence of molecular markers, measure the amount of each of these markers, quantify total cells, populations and subpopulations; it also allows microparticles to be recognized as microvesicles and even nanoparticles. Likewise, the sorting function enables the physical separation of populations based on the 9 parameters, thus obtaining purified cells or clones for cell cultures, proteomic\/gene expression studies or cell therapy.<\/p>\n<p>The characteristics of the platform make it an ideal tool for obtaining a large amount of information in the areas of molecular and cellular biology, pathology, immunology, inflammation, genetics, oncology, hematology, cardiology, neuroscience, among others.[\/et_pb_toggle][et_pb_toggle title=&#8221;Fees&#8221; admin_label=&#8221;Tarifas&#8221; _builder_version=&#8221;4.14.7&#8243; _module_preset=&#8221;default&#8221; body_font=&#8221;|300|||||||&#8221; global_colors_info=&#8221;{}&#8221;]\n<div class=\"wpdt-c row wpDataTableContainerSimpleTable wpDataTables wpDataTablesWrapper\n\"\n    >\n        <table id=\"wpdtSimpleTable-25\"\n           style=\"border-collapse:collapse;\n                   border-spacing:0px;\"\n           class=\"wpdtSimpleTable wpDataTable\"\n           data-column=\"4\"\n           data-rows=\"4\"\n           data-wpID=\"25\"\n           data-responsive=\"0\"\n           data-has-header=\"1\">\n\n                    <thead>        <tr class=\"wpdt-cell-row  remove-borders \" >\n                                <th class=\"wpdt-cell  wpdt-empty-cell  remove-borders \"\n                                            data-cell-id=\"A1\"\n                    data-col-index=\"0\"\n                    data-row-index=\"0\"\n                    style=\" width:25%;                    padding:10px;\n                    \"\n                    >\n                                                            <\/th>\n                                                <th class=\"wpdt-cell wpdt-bc-10294C wpdt-tc-FFFFFF remove-borders \"\n                                            data-cell-id=\"B1\"\n                    data-col-index=\"1\"\n                    data-row-index=\"0\"\n                    style=\" width:25%;                    padding:10px;\n                    \"\n                    >\n                                        IDIS internal fee                    <\/th>\n                                                <th class=\"wpdt-cell wpdt-bc-10294C wpdt-tc-FFFFFF remove-borders \"\n                                            data-cell-id=\"C1\"\n                    data-col-index=\"2\"\n                    data-row-index=\"0\"\n                    style=\" width:25%;                    padding:10px;\n                    \"\n                    >\n                                        External public organizations                    <\/th>\n                                                <th class=\"wpdt-cell wpdt-bc-10294C wpdt-tc-FFFFFF remove-borders \"\n                                            data-cell-id=\"D1\"\n                    data-col-index=\"3\"\n                    data-row-index=\"0\"\n                    style=\" width:25%;                    padding:10px;\n                    \"\n                    >\n                                        External private organizations                    <\/th>\n                                        <\/tr>\n                    <tbody>        <tr class=\"wpdt-cell-row  remove-borders \" >\n                                <td class=\"wpdt-cell wpdt-bold remove-borders \"\n                                            data-cell-id=\"A2\"\n                    data-col-index=\"0\"\n                    data-row-index=\"1\"\n                    style=\"                    padding:10px;\n                    \"\n                    >\n                                        Characterization without CDU                    <\/td>\n                                                <td class=\"wpdt-cell  remove-borders \"\n                                            data-cell-id=\"B2\"\n                    data-col-index=\"1\"\n                    data-row-index=\"1\"\n                    style=\"                    padding:10px;\n                    \"\n                    >\n                                        43,29 \u20ac                    <\/td>\n                                                <td class=\"wpdt-cell  remove-borders \"\n                                            data-cell-id=\"C2\"\n                    data-col-index=\"2\"\n                    data-row-index=\"1\"\n                    style=\"                    padding:10px;\n                    \"\n                    >\n                                        56,28 \u20ac                    <\/td>\n                                                <td class=\"wpdt-cell  remove-borders \"\n                                            data-cell-id=\"D2\"\n                    data-col-index=\"3\"\n                    data-row-index=\"1\"\n                    style=\"                    padding:10px;\n                    \"\n                    >\n                                        90,04 \u20ac                    <\/td>\n                                        <\/tr>\n                            <tr class=\"wpdt-cell-row  remove-borders \" >\n                                <td class=\"wpdt-cell wpdt-bold remove-borders \"\n                                            data-cell-id=\"A3\"\n                    data-col-index=\"0\"\n                    data-row-index=\"2\"\n                    style=\"                    padding:10px;\n                    \"\n                    >\n                                        Characterization with CDU                    <\/td>\n                                                <td class=\"wpdt-cell  remove-borders \"\n                                            data-cell-id=\"B3\"\n                    data-col-index=\"1\"\n                    data-row-index=\"2\"\n                    style=\"                    padding:10px;\n                    \"\n                    >\n                                        45,84 \u20ac                    <\/td>\n                                                <td class=\"wpdt-cell  remove-borders \"\n                                            data-cell-id=\"C3\"\n                    data-col-index=\"2\"\n                    data-row-index=\"2\"\n                    style=\"                    padding:10px;\n                    \"\n                    >\n                                        59,20 \u20ac                    <\/td>\n                                                <td class=\"wpdt-cell  remove-borders \"\n                                            data-cell-id=\"D3\"\n                    data-col-index=\"3\"\n                    data-row-index=\"2\"\n                    style=\"                    padding:10px;\n                    \"\n                    >\n                                        95,36 \u20ac                    <\/td>\n                                        <\/tr>\n                            <tr class=\"wpdt-cell-row  remove-borders \" >\n                                <td class=\"wpdt-cell wpdt-bold remove-borders \"\n                                            data-cell-id=\"A4\"\n                    data-col-index=\"0\"\n                    data-row-index=\"3\"\n                    style=\"                    padding:10px;\n                    \"\n                    >\n                                        Data analysis\/ reporting\/ consultancy                    <\/td>\n                                                <td class=\"wpdt-cell  remove-borders \"\n                                            data-cell-id=\"B4\"\n                    data-col-index=\"1\"\n                    data-row-index=\"3\"\n                    style=\"                    padding:10px;\n                    \"\n                    >\n                                        16,81 \u20ac                    <\/td>\n                                                <td class=\"wpdt-cell  remove-borders \"\n                                            data-cell-id=\"C4\"\n                    data-col-index=\"2\"\n                    data-row-index=\"3\"\n                    style=\"                    padding:10px;\n                    \"\n                    >\n                                        21,85 \u20ac                    <\/td>\n                                                <td class=\"wpdt-cell  remove-borders \"\n                                            data-cell-id=\"D4\"\n                    data-col-index=\"3\"\n                    data-row-index=\"3\"\n                    style=\"                    padding:10px;\n                    \"\n                    >\n                                        34,96 \u20ac                    <\/td>\n                                        <\/tr>\n                    <\/table>\n<\/div><style id='wpdt-custom-style-25'>\n.wpdt-bc-10294C { background-color: #10294C !important;}\n.wpdt-tc-FFFFFF { color: #FFFFFF !important;}\n<\/style>\n<style>\n                    \n                                                \n                <\/style>\n<\/p>\n<p>VAT not included[\/et_pb_toggle][\/et_pb_column][et_pb_column type=&#8221;1_3&#8243; _builder_version=&#8221;4.10.7&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_text admin_label=&#8221;Contacto&#8221; _builder_version=&#8221;4.14.1&#8243; _module_preset=&#8221;default&#8221; text_text_color=&#8221;#FFFFFF&#8221; header_text_color=&#8221;#FFFFFF&#8221; header_2_text_align=&#8221;left&#8221; header_2_text_color=&#8221;#FFFFFF&#8221; background_color=&#8221;#10294c&#8221; custom_margin=&#8221;0px||||false|false&#8221; custom_padding=&#8221;6px||0px|6px|false|false&#8221; locked=&#8221;on&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<h2>Contact<\/h2>\n<p>[\/et_pb_text][et_pb_text _builder_version=&#8221;4.14.7&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;]<strong>Pablo Hervella Lorenzo<\/strong><\/p>\n<p><a href=\"mailto:Pablo.Hervella.Lorenzo@sergas.es\">Pablo.Hervella.Lorenzo@sergas.es<\/a><\/p>\n<p>&nbsp;[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=&#8221;1&#8243; admin_label=&#8221;Logos_internas&#8221; _builder_version=&#8221;4.14.7&#8243; _module_preset=&#8221;default&#8221; background_color=&#8221;RGBA(255,255,255,0)&#8221; custom_margin=&#8221;5px|||||&#8221; custom_padding=&#8221;27px||0px|||&#8221; da_disable_devices=&#8221;off|off|off&#8221; global_module=&#8221;11879&#8243; saved_tabs=&#8221;all&#8221; locked=&#8221;off&#8221; collapsed=&#8221;on&#8221; global_colors_info=&#8221;{}&#8221; da_is_popup=&#8221;off&#8221; da_exit_intent=&#8221;off&#8221; da_has_close=&#8221;on&#8221; da_alt_close=&#8221;off&#8221; da_dark_close=&#8221;off&#8221; da_not_modal=&#8221;on&#8221; da_is_singular=&#8221;off&#8221; da_with_loader=&#8221;off&#8221; da_has_shadow=&#8221;on&#8221;][et_pb_row column_structure=&#8221;1_4,1_4,1_4,1_4&#8243; 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