E054 – Epitranscriptomics and aging

Objectives

Our research group is focused on identifying new molecular mechanisms involved in defining cell identity, both in physiological and pathological models. In particular, we are interested in studying the role of RNA modifications (Epitranscriptomic) and their interrelation with chromatin modifications (Epigenetic) in the biology of pluripotent cells, during aging and in cell rejuvenation.

Lines of research

• Epigenetic and epitranscriptomic mechanisms involved in aging and associated diseases.
• Impact of the hm5C RNA modification on cell aging in the context of obesity.
• Function of TET2 in RNA in cell aging and rejuvenation.

• Covelo-Molares H, Souto Y, Fidalgo M, Guallar D. A Simple, Rapid, and Cost-Effective Method for Loss-of-Function Assays in Pluripotent Cells. Methods Mol Biol. 2021 Oct 6. doi: 10.1007/7651_2021_434.
• Wang J, Yu H, Ma Q, Zeng P, Wu D, Hou Y, Liu X, Jia L, Sun J, Chen Y, Guallar D, Fidalgo M, Chen J, Yu Y, Jiang S, Li F, Zhao C, Huang X, Wang J, Li C, Sun Y, Zeng X, Zhang W, Miao Y, Ding J. Phase separation of OCT4 controls TAD reorganization to promote cell fate transitions. Cell Stem Cell. 2021 Oct 7;28(10):1868-1883.e11. doi: 10.1016/j.stem.2021.04.023. Epub 2021 May 25.
• Garcia-Outeiral V, De La Parte C, Fidalgo M, Guallar D. The Complexity of TET2 Functions in Pluripotency and Development. Frontiers in Cell and Developmental Biology. 2021 Jan 18. doi:10.3389/fcell.2020.630754.
• Lan J, Rajan N, Bizet M, Penning A, Singh NK, Guallar D, Calonne E, Li Greci A, Bonvin E, Deplus R, Hsu PJ, Nachtergaele S, Ma C, Song R, Fuentes-Iglesias A, Hassabi B, Putmans P, Mies F, Menschaert G, Wong JJL, Wang J, Fidalgo M, Yuan B, Fuks F. Functional role of Tet-mediated RNA hydroxymethylcytosine in mouse ES cells and during differentiation. Nature Communications 2020 Oct 2;11(1):4956. doi: 10.1038/s41467-020-18729-6.
• Fuentes-Iglesias A, Garcia-Outeiral V, Pardavila JA, Wang J*, Fidalgo M*, Guallar D*. An Optimized Immunoprecipitation Protocol for Assessing Protein-RNA Interactions In Vitro. STAR Protocols 2020 Sep 18;1(2):100093. doi: 10.1016/j.xpro.2020.100093. (*Co-corresponding authors)
• Guallar D*,#, Fuentes-Iglesias A#, Souto Y, Ameneiro C, Freire-Agulleiro O, Pardavila JA, Escudero A, Garcia-Outeiral V, Moreira T, Saenz C, Xiong H, Liu D, Xiao S, Hou Y, Wu K, Torrecilla D, Hartner JC, Blanco MG, Lee LJ, López M, Walkley CR, Wang J*, Fidalgo M*. ADAR1-Dependent RNA Editing Promotes MET and iPSC Reprogramming by Alleviating ER Stress. Cell Stem Cell. 2020 Aug 6;27(2):300-314.e11. doi: 10.1016/j.stem.2020.04.016. (#Co-first, *Co-corresponding author).
• Ameneiro C, Moreira T, Fuentes-Iglesias A, Coego A, Garcia-Outeiral V, Escudero A, Torrecilla D, Mulero-Navarro S, Carvajal-Gonzalez JM, Guallar D*, Fidalgo M*. BMAL1 coordinates energy metabolism and differentiation of pluripotent stem cells. Life Science Alliance. 2020 Apr 13;3(5):e201900534. doi: 10.26508/lsa.201900534.*Co-corresponding authors.
• Folgueira C, Beiroa D, Porteiro B, Duquenne M, Puighermanal E, Fondevila MF, Barja-Fernández S, Gallego R, Hernández-Bautista R, Castelao C, Senra A, Seoane P, Gómez N, Aguiar P, Guallar D, Fidalgo M, Romero-Pico A, Adan R, Blouet C, Labandeira-García JL, Jeanrenaud F, Kallo I, Liposits Z, Salvador J, Prevot V, Dieguez C, Lopez M, Valjent E, Frühbeck G, Seoane LM, Nogueiras R. Hypothalamic dopamine signaling regulates brown fat thermogenesis. Nature Metabolism. 2019 Aug;1(8):811-829. doi: 10.1038/s42255-019-0099-7.

https://www.usc.es/cimus/es/investigacion/grupos-de-investigacion/epitranscriptomics-ageing