Proteomics

COMMON SUPPORT PLATFORMS

Equipment

 

Description

​The Proteomics platform was created with the purpose of boosting research, providing support and offering researchers a comprehensive infrastructure in the field of proteomics managed by highly specialised staff.

It has cutting-edge equipment used to conduct complete proteome characterization studies and also differential expression analysis studies. Since the cause of most human diseases is the functional deregulation of protein interaction, the most important clinical application is the search and subsequent identification of biomarkers of the different pathologies.

The work of the Platform is focused on the separation, identification, quantification and characterization of proteins in biological systems by means of multidimensional liquid chromatography and mass-spectrometry-based proteomics analysis. Our laboratory is specialized in a wide range of approaches to support proteome-related studies, from the identification and quantification of individual proteins, to the complex analysis of samples in different biological systems. In addition to this, the platform collaborates with other groups in the research and search of appropriate experimental designs, assisting in the writing of papers, analysis and result validation, among other tasks.

Moreover, in addition to our main line of work as a service, we also carry out our own research, developing projects focused on protocol optimization, equipment updating and the introduction of new techniques to offer the most comprehensive and innovative services.

Services
  • Determination of Molecular Masses (DDM)
  • Identification of proteins by peptide-mass fingerprinting (MALDI-TOF)
  • Identification of protein mixtures by nanoLC-MALDI-TOF/TOF
  • Massive identification of proteins by LC-MSMS methods (DDA methods using Triple TOF equipment)
  • Reversed-phase chromatography (Nano-HPLC)
  • Separation of proteins by mono-dimensional electrophoresis (SDS-PAGE)
  • Separation of proteins by bi-dimensional electrophoresis (2-DE)
  • Differential proteomics with 2D-DIGE, ITRAQ, SILAC labelling
  • SWATH label-free differential proteomics
  • Post-translational Modifications (PTM): phosphorylation, glycosylation, glycation…
  • Use of different programmes for data analysis: ProteinPilot for protein identification; Peak View, Marker View and Skyline for protein quantification… PEAKS for the study of post-translational modifications and De Novo sequencing
  • Other services:
    • Gel scanning
    • Sample preparation
    • Advice on the experimental design of proteomic analyses
    • Data analysis in computer databases
Fees
Fees IDIS Public organizations Private organizations
Sample preparation-precipitation (per sample) 11,7 € 15,3 € 17,6 €
Preparation-desalting/concentration reverse phase (C18) 12,6 € 16,3 € 18,9 €
Preparation-desalting/concentration centrifugation (CENTRICON) 13,5 € 17,6 € 20,3 €
Preparation-protein quantification (RC-DC) 30,3 € 39,4 € 45,4 €
Depletion 22,1 € 28,8 € 33,2 €
Solution digestion 22,6 € 29,4 € 33,9 €
Gel digestion 22,7 € 29,6 € 34,1 €
Determination of molecular masses 9,0 € 11,7 € 13,6 €
Identification by peptide-mass fingerprinting-up to 10 samples 19,1 € 24,8 € 28,6 €
Identification by peptide-mass fingerprinting- from 11 to 50 samples 16,9 € 22,0 € 25,4 €
Identification by peptide-mass fingerprinting-more than 51 samples 16,7 € 21,7 € 25,0 €
Fragmentation MS/MS 2,1 € 2,7 € 3,2 €
De Novo sequencing-MALDI 71,1 € 92,5 € 106,7 €
De Novo sequencing - Triple TOF 146,8 € 190,9 € 220,3 €
LC-MS/MS TRIPLE TOF - Ultrashort gradient (15 minutes) 44,5 € 52,9 € 61,1 €
LC-MS/MS TRIPLE TOF - Short gradient (40 minutes) 86,1 € 141,4 € 163,2 €
LC-MS/MS TRIPLE TOF - Medium gradient (75 minutes) 126,7 € 221,1 € 255,1 €
LC-MS/MS TRIPLE TOF - Long gradient (95 minutes) 183,5 € 465,6 € 537,2 €
MRM 163,8 € 212,9 € 245,7 €
DIGE - Quantitative proteomics 278,3 € 361,8 € 417,4 €
Bidimensional electrophoresis
2D-PAGE (7cm) 131,0 € 170,3 € 196,5 €
2D-PAGE (from 11 to 21 cm) 24,1 € 31,3 € 36,1 €
Coomassie staining 2,5 € 3,2 € 3,7 €
Sypro staining 10,3 € 13,4 € 15,5 €
Silver staining 3,9 € 5,1 € 5,8 €
Data analysis
Sample processing (hour) 19,7 € 25,7 € 29,6 €

VAT not included.

Contacto

Susana B. Bravo López

Susana.Belen.Bravo.Lopez@sergas.es