Proteomics

​The Proteomics platform was created with the purpose of boosting research, providing support and offering researchers a comprehensive infrastructure in the field of proteomics managed by highly specialised staff.

It has cutting-edge equipment used to conduct complete proteome characterization studies and also differential expression analysis studies. Since the cause of most human diseases is the functional deregulation of protein interaction, the most important clinical application is the search and subsequent identification of biomarkers of the different pathologies.

The work of the Platform is focused on the separation, identification, quantification and characterization of proteins in biological systems by means of multidimensional liquid chromatography and mass-spectrometry-based proteomics analysis. Our laboratory is specialized in a wide range of approaches to support proteome-related studies, from the identification and quantification of individual proteins, to the complex analysis of samples in different biological systems. In addition to this, the platform collaborates with other groups in the research and search of appropriate experimental designs, assisting in the writing of papers, analysis and result validation, among other tasks.

Moreover, in addition to our main line of work as a service, we also carry out our own research, developing projects focused on protocol optimization, equipment updating and the introduction of new techniques to offer the most comprehensive and innovative services.

• Determination of Molecular Masses (DDM)
• Identification of proteins by peptide-mass fingerprinting (MALDI-TOF)
• Identification of protein mixtures by nanoLC-MALDI-TOF/TOF
• Massive identification of proteins by LC-MSMS methods (DDA methods using Triple TOF equipment)
• Reversed-phase chromatography (Nano-HPLC)
• Separation of proteins by mono-dimensional electrophoresis (SDS-PAGE)
• Separation of proteins by bi-dimensional electrophoresis (2-DE)
• Differential proteomics with 2D-DIGE, ITRAQ, SILAC labelling
• SWATH label-free differential proteomics
• Post-translational Modifications (PTM): phosphorylation, glycosylation, glycation…
• Use of different programmes for data analysis: ProteinPilot for protein identification; Peak View, Marker View and Skyline for protein quantification… PEAKS for the study of post-translational modifications and De Novo sequencing
• Other services:
  • Gel scanning
  • Sample preparation
  • Advice on the experimental design of proteomic analyses
  • Data analysis in computer databases

VAT not included.

• Nóvoa E, da Silva Lima N, Gonzalez-Rellan MJ, Chantada-Vazquez MDP, Verheij J, Rodriguez A, Esquinas-Roman EM, Fondevila MF, Koning M, Fernandez U, Cabaleiro A, Parracho T, Iglesias-Moure J, Seoane S, Porteiro B, Escudero A, Senra A, Perez-Fernandez R, López M, Fidalgo M, Guallar D, Martinez-Chantar ML, Dieguez C, Varela-Rey M, Prevot V, Schwaninger M, Meijnikman A, Bravo SB, Frühbeck G, Nogueiras R. Mitochondrial antiviral signaling protein enhances MASLD progression via the ERK/TNFα/NFκβ pathway. Hepatology. 2024 May 16. doi: 10.1097/HEP.0000000000000930. Epub ahead of print. PMID: 38761407.
• Rodrigues JS, Chenlo M, Bravo SB, Perez-Romero S, Suarez-Fariña M, Sobrino T, Sanz-Pamplona R, González-Prieto R, Blanco Freire MN, Nogueiras R, López M, Fugazzola L, Cameselle-Teijeiro JM, Alvarez CV. dsRNAi-mediated silencing of PIAS2beta specifically kills anaplastic carcinomas by mitotic catastrophe. Nat Commun. 2024 May 14;15(1):3736. doi: 10.1038/s41467-024-47751-1. PMID: 38744818; PMCID: PMC11094195.
• Feás X, Alonso-Sampedro M, Bravo SB, Vidal C. Peeking into the Stingers: A Comprehensive SWATH-MS Study of the European Hornet Vespa crabro (Linnaeus, 1758) (Hymenoptera: Vespidae) Venom Sac Extracts. Int J Mol Sci. 2024 Mar 28;25(7):3798. doi: 10.3390/ijms25073798. PMID: 38612607; PMCID: PMC11011553.
• Álvarez JV, Bravo SB, Chantada-Vázquez MP, Pena C, Colón C, Tomatsu S, Otero- Espinar FJ, Couce ML. Morquio A Syndrome: Identification of Differential Patterns of Molecular Pathway Interactions in Bone Lesions. Int J Mol Sci. 2024 Mar 12;25(6):3232. doi: 10.3390/ijms25063232. PMID: 38542208; PMCID: PMC10970612.
• Aragón-Herrera A, Feijóo-Bandín S, Vázquez-Abuín X, Anido-Varela L, Moraña-Fernández S, Bravo SB, Tarazón E, Roselló-Lletí E, Portolés M, García-Seara J, Seijas J, Rodríguez-Penas D, Bani D, Gualillo O, González-Juanatey JR, Lago F. Human recombinant relaxin-2 (serelaxin) regulates the proteome, lipidome, lipid metabolism and inflammatory profile of rat visceral adipose tissue. Biochem Pharmacol. 2024 May;223:116157. doi: 10.1016/j.bcp.2024.116157. Epub 2024 Mar 20. PMID: 38518995.
• López-Valverde L, Vázquez-Mosquera ME, Colón-Mejeras C, Bravo SB, Barbosa-Gouveia S, Álvarez JV, Sánchez-Martínez R, López-Mendoza M, López-Rodríguez M, Villacorta-Argüelles E, Goicoechea-Diezhandino MA, Guerrero-Márquez FJ, Ortolano S, Leao-Teles E, Hermida-Ameijeiras Á, Couce ML. Characterization of the plasma proteomic profile of Fabry disease: Potential sex- and clinical phenotype-specific biomarkers. Transl Res. 2024 Jul;269:47-63. doi: 10.1016/j.trsl.2024.02.006. Epub 2024 Feb 21. PMID: 38395389.
• Pazos-Pérez A, Piñeiro-Ramil M, Franco-Trepat E, Alonso-Pérez A, Guillán-Fresco M, Crespo-Golmar A, López-Fagúndez M, Aranda JC, Bravo SB, Jorge-Mora A, Gómez R. The Hepatokine RBP4 Links Metabolic Diseases to Articular Inflammation. Antioxidants (Basel). 2024 Jan 19;13(1):124. doi: 10.3390/antiox13010124. PMID: 38275649; PMCID: PMC10812991.
• García-Vega D, Sánchez-López D, Rodríguez-Carnero G, Villar-Taibo R, Viñuela JE, Lestegás-Soto A, Seoane-Blanco A, Moure-González M, Bravo SB, Fernández ÁL, González-Juanatey JR, Eiras S. Semaglutide modulates prothrombotic and
  atherosclerotic mechanisms, associated with epicardial fat, neutrophils and endothelial cells network. Cardiovasc Diabetol. 2024 Jan 3;23(1):1. doi: 10.1186/s12933-023-02096-9. PMID: 38172989; PMCID: PMC10765851.